DEPARTAMENTO DE BIOTECNOLOGIA

Dra. Keiko Shirai
Profesor Titular "C". Tiempo completo Laboratorio de Biopolímeros Edificio S, Primer piso 157
Tel. +(52) 55-58-04-4921
smk@xanum.uam.mx

Estudios

Licenciatura Ingeniería Bioquímica Industrial, Universidad Autónoma Metropolitana.
Especialización, Universidad de Loughborough Reino Unido.
Maestría en Biotecnología, Universidad Autónoma Metropolitana.
Doctorado en Ciencias Biológicas, Universidad Autónoma Metropolitana.

Enzimología y Fermentaciones

Actualmente imparte los cursos:

http://docencia.izt.uam.mx/smk/

Líneas principales de investigación.

• Obtención de compuestos con valor agregado a partir de desechos de crustáceos mediante microorganismos y enzimas
• Desarrollo de estrategias de producción de enzimas con uso potencial en biocontrol y obtención de oligosacáridos.

chitin, chitosan, chitinases, lactic acid bacteria, fungi, submerged fermentations, solid state fermentation, shrimp wastes, N-acetylglucosamine, glucosamine, oligosaccharides.

Publicaciones Recientes (2002-2010)

• Gustavo Martínez-Castellanos, Clara Pelayo-Zaldívar, Laura J. Pérez-Flores, Alberto López-Luna, Miquel Gimeno, Eduardo Bárzana, Keiko Shirai. Postharvest Litchi (Litchi chinensis Sonn.) Quality Preservation by Lactobacillus plantarum. En prensa. 2010. Postharvest Biology and Technology.
• Milton Spanopoulos-Hernandez, Jesús T. Ponce-Palafox, Guillermo Barba Quintero, Jorge R. Ruelas-Inzunza, María R. Tiznado Contreras, Crisantema Hernández González, Keiko Shirai. Producción de ensilados biológicos a partir de desechos de pescado, del ahumado de atún aleta amarilla (Thunnus albacares) y del fileteado de tilapia (Oreochromis sp), para la alimentación de especies acuícolas. 2010. Revista Mexicana de Ingenieria Quimica 9(2):167-178.
• Laura Ramirez-Coutiño, Jesús Espinosa-Marquez, Martin G. Peter, Keiko Shirai. The effect of pH on the production of chitinolytic enzymes of Verticillium fungicola in submerged cultures. 2010. Bioresource Technology 101 (23) 9236-9240.
• Diana Alonso, Miquel Gimeno, José D. Sepúlveda-Sánchez, and Keiko Shirai. Chitosan-based microcapsules containing grapefruit seed extract grafted onto cellulose fibres by a non-toxic procedure. 2010. Carbohydrate Research 345(6):854-859.
• Jacob-Lopes, E., Revah, S., Hernández, S., Shirai, K., Franco, T.T. Development of operational strategies to remove carbon dioxide in photobioreactors. 2009. Chemical Engineering Journal 153 (1-3), pp. 120-126.
• Jose L. Davila-Rodriguez, Vladimir A. Escobar-Barrios, Keiko Shirai, Jose R. Rangel-Mendez. Synthesis of a chitin-based biocomposite for water treatment: Optimization for fluoride removal. 2009. Journal of Fluorine Chemistry 130: 718–726.
• Gabriel Vigueras, Sonia Arriaga, Keiko Shirai, Marcia Morales, Sergio Revah. Hydrophobic response of the fungus Rhinocladiella similis in the biofiltration with volatile organic compounds with different polarity. 2009. Biotechnology letters 31:1203-1209.
• Diana Alonso, Miquel Gimeno, Roberto Olayo, Humberto Vázquez-Torres, José D. Sepúlveda-Sánchez, and Keiko Shirai. Cross-linking Chitosan into UV-irradiated Cellulose Fibers for the Preparation of Antimicrobial-finished Textiles. 2009. Carbohydrate Polymers, 77(3):536–543.
• Gustavo Martínez-Castellanos, Keiko Shirai*, Clara Pelayo-Zaldivar, Laura J. Pérez-Flores, José D. Sepúlveda-Sánchez. Effect of Lactobacillus plantarum and chitosan in the reduction of browning of pericarp Rambutan (Nephelium lappaceum). 2009. Food Microbiology, 26(4):444-449.
• Pacheco, Neith; Gárnica-González, Monica; Ramirez-Hernandez, Jessica; Flores-Albino, Belem; Gimeno, Miquel; Bárzana, Eduardo; Shirai, Keiko. Effect of temperature on chitin and astaxanthin recoveries from shrimp waste using lactic acid bacteria. 2009. Bioresource Technology, 100(11):2849-2854.
• J. R. Rangel-Mendez, R. Monroy-Zepeda, E. Leyva-Ramos, P.E. Díaz-Flores, K Shirai.Chitosan selectivity for removing Cadmium (ii), Copper (ii), and Lead (ii) from aqueous phase: pH and organic matter effect. (2009) Journal of Hazardous Materials, 162:503–511.
• Zaizy Rocha-Pino, Keiko Shirai, Ladislao Arias, Humberto Vazquez-Torres. Efecto de la calidad del agua y tamaño de partícula en la producción de quitosano a partir de beta-quitina extraída de desperdicios de calamar gigante (Dosidicus gigas). Revista Mexicana de Ingenieria Quimica 7(3):299-307.
• José Carmen Ramírez-Ramírez, Sergio Huerta, Ladislao Arias, Arely Prado, Ramon Soriano and Keiko Shirai. Utilization of fisheries by-catch and processing wastes for lactic acid fermented silage and evaluation of degree of protein hydrolysis and in vitro digestibility. 2008. Revista Mexicana de Ingenieria Quimica 7(3):195-204.
• C.P. Larralde-Corona, M.R. Santiago-Mena, A.M. Sifuentes-Rincón, I.C. Rodríguez-Luna, M.A. Rodríguez-Pérez, K. Shirai and J.A. Narváez-Zapata. Biocontrol potential and polyphasic characterization of novel native Trichoderma strains against Macrophomina phaseolina isolated from sorghum and common bean. 2008. Applied Microbiology and Biotechnology. 80(1): 167-77.
• Gabriel Vigueras, Keiko Shirai, Daniel Martins, Telma Teixeira Franco, Luciana Fleuri Sergio Revah. Toluene gas phase biofiltration by Paecilomyces lilacinus and identification of a hydrophobin protein from produced thereof. 2008. Applied Microbiology and Biotechnology. 80:147-154.
• Neith Pacheco, C. Patricia Larralde-Corona, Jose Sepulveda, Stéphan Trombotto, Alain Domard and Keiko Shirai. Evaluation of chitosans and Pichia guillermondii as growth inhibitors of Penicillium digitatum. 2008. International Journal of Biological Macromolecules 43:20–26.
• María del Carmen Marin-Cervantes, Yoyi Matsumoto, Laura Ramírez-Coutiño, Zaizy Rocha-Pino, Gustavo Viniegra and Keiko Shirai.Effect of moisture content in polyurethane foams as support for solid-substrate fermentation of Lecanicillium lecanii on the production profiles of chitinases. 2008. Process Biochemistry. 43 (1): 24–32
• Miquel Gimeno, César Martínez-Ibarra, Neith Pacheco, Roeb García-Arrazola, Eduardo Bárzana and Keiko Shirai.One-solvent Extraction of Astaxanthin from Lactic acid Fermented Shrimp Wastes. 2007. Journal of Agricultural and Food Chemistry. 55:10345–10350
• Keiko Shirai. Fungal chitinases. In: Advances in Agricultural and Food Biotechnology, 2006: ISBN: 81-7736-269-0. Editors: Ramón Gerardo Guevara-González and Irineo Torres-Pacheco. Research Signpost 37/661 (2), Fort P.O., Trivandrum-695 023, Kerala, India.
• Laura Ramírez-Coutiño, María del Carmen Marín-Cervantes, Sergio Huerta, Sergio Revah y Keiko Shirai. Enzymatic hydrolysis of chitin in the production of oligosaccharides using Lecanicillium fungicola chitinases. Process Biochemistry 41 (2006) 1106–1110.
• Yoyi Matsumoto, Gerardo Saucedo, Sergio Revah y Keiko Shirai “Production of beta-N-acetylhexosaminidase of Verticillium lecanii by solid state and submerged fermentations utilizing shrimp waste silage as substrate and inducer”. 2004. Process Biochemistry 39(6):665-6714.
• Maribel Plascencia Jatomea, Gustavo Viniegra, Roberto Olayo, Mónica Castillo y Keiko Shirai “Effect of chitosan and temperature on spore germination of Aspergillus niger”. 2003. Macromolecular Bioscience 3:582–586.
• Maribel Plascencia-Jatomea, Miguel A. Olvera-Novoa, José Luis Arredondo-Figueroa, George, M.Hall y Keiko Shirai. "Feasibility of fishmeal replacement by shrimp-head silage protein hydrolysate in Nile tilapia (Oreochromis niloticus (L)) diets". 2002. Journal of the Science of Food and Agriculture 82:753-759.
• Luis A. Cira, Sergio Huerta, George M. Hall y Keiko Shirai. "Pilot scale lactic acid fermentation of shrimp wastes for chitin recovery" 2002. Process Biochemistry 37:1359-1366.

Keiko Shirai y Diana Alonso Segura. "Proceso para la funcionalización de textiles de celulosa mediante la fijación de microcápsulas que contienen aceites esenciales". Presentada por Instituto Mexicano de Propiedad Industrial (IMPI)

Keiko Shirai, Sergio Huerta, Maribel Plascencia, Luis, Cira. "Proceso de Aprovechamiento de Desperdicios de Camarón Para Obtención de Quitina, Proteínas, Calcio y Pigmentos" Otorgada por el Instituto Mexicano de Propiedad Industrial (IMPI).